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B-hNECTIN4 MC38
Common name
B-hNECTIN4 MC38 Catalog number     310954
Aliases EDSS1, LNIR, PRR4, PVRL4, NECTIN4 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Nectin4 gene was replaced by human NECTIN4 coding sequence in B-hNECTIN4 MC38 cells. Human NECTIN4 is highly expressed on the surface of B-hNECTIN4 MC38 cells.

Application

B-hNECTIN4 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hNECTIN4 MC38 cells. The exogenous promoter and human NECTIN4 coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Nectin4 gene, resulting in a non-functional transcript.

Protein expression analysis 



NECTIN4 expression analysis in B-hNECTIN4 MC38 cells by flow cytometry. Single cell suspensions from B-hNECTIN4 MC38 cultures were stained with species-specific anti-NECTIN4 antibody. Human NECTIN4 was detected on the surface of B-hNECTIN4 MC38 cells but not wild-type MC38 cells. The 3-H03 clone of B-hNECTIN4 MC38 cells was used for in vivo experiments.

Protein expression analysis of tumor cells


B-hNECTIN4 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5), and on 35 days post inoculation, tumor cells were harvested and assessed for human NECTIN4 expression by flow cytometry. As shown, human NECTIN4 was highly expressed on the surface of tumor cells. Therefore, B-hNECTIN4 MC38 cells can be used for in vivo efficacy studies of novel NECTIN4 therapeutics.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hNECTIN4 MC38 cells. B-hNECTIN4 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hNECTIN4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hNECTIN4 MC38 cells. B-hNECTIN4 MC38 cells (5x105, 1x106, 5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hCD3E mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hNECTIN4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes


from clipboard

Subcutaneous homograft tumor growth of B-hNECTIN4 MC38 cells. B-hNECTIN4 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hNECTIN4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.