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B-hLILRB4 luc EL4
Common name
B-hLILRB4 luc EL4 Catalog number     111915
Aliases / Disease   Lymphoma
Organism
Mouse
Strain  C57BL/6
Tissue types Lymphoma Tissue  T lymphocyte

Description



The human LILRB4(CD85K) coding sequence was inserted to mouse Hipp11(H11) allele. Human LILRB4 is highly expressed on the surface of B-hLILRB4 luc EL4 cells.



Application


B-hLILRB4 luc EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.



Phenotypic Analysis



The luminescence signal intensity of B-hLILRB4 luc EL4 cells. The luminescence intensity was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610), and B-hLILRB4 luc EL4 cells have strong luminescence signal.


Protein expression analysis




LILRB4 expression analysis in B-hLILRB4 luc EL4 cells by flow cytometry. Single cell suspensions from B-hLILRB4 luc EL4 cultures were stained with species-specific anti-LILRB4 antibody. Human LILRB4 was detected on the surface of B-hLILRB4 luc EL4 cells but not wild-type EL4 cells. The 10-G04 clone of B-hLILRB4 luc EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes




Tumor growth and in vivo imaging of B-hLILRB4 luc EL4. B-hLILRB4 luc EL4 cells (2x105) were  injected by tail vein into C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed on days 3, 7, 10, 14, and 17, (B) Mice body weight (Mean ± SEM). B-hLILRB4 luc EL4 cells can be used for in vivo efficacy evaluation.


Tumor growth curve & Body weight changes


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Subcutaneous homograft tumor growth of B-hLILRB4 luc EL4 cells. B-hLILRB4 luc EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLILRB4 luc EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.