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B-hJAM3 MC38
Common name
B-hJAM3 MC38 Catalog number    322013
Aliases

JAM3, JAM-2, JAM-3, JAM-C,

JAMC,CD323, junctional adhesion molecule 3

Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 

The mouse Jam3 gene was replaced by human JAM3 coding sequence in B-hJAM3 MC38 cells. Human JAM3 is highly expressed on the surface of B-hJAM3 MC38 cells.


Application

B-hJAM3 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Protein expression analysis 

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JAM3 expression analysis in B-hJAM3 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hJAM3 MC38 cultures were stained with species-specific anti-JAM3 antibody. Human JAM3 was detected on the surface of B-hJAM3 MC38 cells but not wild-type MC38 cells. The 2-H12 clone of B-hJAM3 MC38 cells was used for in vivo tumor growth assays.


Tumor growth curve & Body weight changes


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Subcutaneous homograft tumor growth of B-hJAM3 MC38 cells. B-hJAM3 MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hJAM3 MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-hJAM3 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6), and on 35 days post inoculation, tumor cells were harvested and assessed for human JAM3 expression by flow cytometry. As shown, human JAM3 was highly expressed on the surface of tumor cells. Therefore, B-hJAM3 MC38 cells can be used for in vivo efficacy studies of JAM3 therapeutics.