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B-hGPRC5D MC38
Common name
B-hGPRC5D MC38 Catalog number     321822
Aliases GPRC5D, G protein-coupled receptor class C group 5 member D  Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 



The mouse Gprc5d gene was replaced by human GPRC5D coding sequence in B-hGPRC5D MC38 cells. Human GPRC5D is highly expressed on the surface of B-hGPRC5D MC38 cells.


Application


B-hGPRC5D MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Protein expression analysis




GPRC5D expression analysis in B-hGPRC5D MC38 cells by flow cytometry.

Single cell suspensions from wild-type MC38 and B-hGPRC5D MC38 cultures were stained with species-specific anti-GPRC5D antibody. Human GPRC5D was detected on the surface of B-hGPRC5D MC38 cells but not wild-type MC38 cells. The 3-D10 clone of B-hGPRC5D MC38 cells was used for in vivo tumor growth assays.



Tumor growth curve & Body weight changes



Subcutaneous homograft tumor growth of B-hGPRC5D MC38 cells.

B-hGPRC5D MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGPRC5D MC38 cells were able to form tumors in vivo and can be used for efficacy studies.


Protein expression analysis of tumor cells


from clipboard

B-hGPRC5D MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6), and on 32 days post inoculation, tumor cells were harvested and assessed for human GPRC5D expression by flow cytometry. As shown, human GPRC5D was highly expressed on the surface of tumor cells. Therefore, B-hGPRC5D MC38 cells can be used for in vivo efficacy studies of GPRC5D therapeutics.

Tumor growth curve & Body weight changes

from clipboard

Subcutaneous homograft tumor growth of B-hGPRC5D MC38 cells. B-hGPRC5D MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGPRC5D MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.