||B-hCLDN18.2 A549||Catalog number||310791|
The human CLDN18.2 coding sequence was inserted into the AAVS1 locus in A549 cells. Human CLDN18.2 is highly expressed on the surface of B-hCLDN18.2 A549 cells.
B-hCLDN18.2 A549 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Gene targeting strategy for B-hCLDN18.2 A549 cells. The exogenous promoter and human CLDN18.2 coding sequence was inserted into the AAVS1 locus wild-type A549.
Tumor growth curve & Body weight changes
Subcutaneous heterograft tumor growth of B-hCLDN18.2 A549 cells. B-hCLDN18.2 A549 cells (1x107) and wild-type A549 cells (1x107) were subcutaneously implanted into B-NDG mice (female, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCLDN18.2 A549 cells were able to establish tumors in vivo and can be used for efficacy studies.