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B-hCCL2 MC38
Common name
B-hCCL2 MC38 Catalog number    321866
Aliases HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30 Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description

The mouse Ccl2 gene was replaced by human CCL2 coding sequence in B-hCCL2 MC38 cells. Human CCL2 can be secreted by B-hCCL2 MC38 cells.


Application


B-hCCL2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hCCL2 MC38 cells. The exogenous promoter and human CCL2 coding sequence was inserted to replace part of murine exon 2~3. The insertion disrupts the endogenous murine Ccl2 gene, resulting in a non-functional transcript.


Protein expression analysis

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CCL2 expression analysis in B-hCCL2 MC38 cells by ELISA. Human CCL2 was detected in the supernatant of B-hCCL2 MC38 cells but not wild-type MC38 cells. Mouse Ccl2 was not detected in the supernatant of wild-type MC38 cells. The 2-D07 clone of B-hCCL2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCCL2 MC38 cells. B-hCCL2 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCCL2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.