B-hCAXII MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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B-hCAXII MC38

Common name	B-hCAXII MC38	Catalog number	321939
Aliases	CA12; CA-XII; T18816; HsT18816	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Car12 gene was replaced by human CAXII (CA12) coding sequence in B-hCAXII MC38 cells. Human CAXII is highly expressed on the surface of B-hCAXII MC38 cells.

Application

B-hCAXII MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hCAXII MC38 cells. The exogenous promoter and human CAXII coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Car12 gene, resulting in a non-functional transcript.

Protein expression analysis

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CAXII expression analysis in B-hCAXII MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCAXII MC38 cultures were stained with species-specific anti-CAXII antibody. Human CAXII was detected on the surface of B-hCAXII MC38 cells but not wild-type MC38 cells. The 2-D05 clone of B-hCAXII MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCAXII MC38 cells. B-hCAXII MC38 cells (5x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 11-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCAXII MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

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B-hCAXII MC38 tumor cells growth of individual mice. B-hCAXII MC38 cells (5x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 11-week-old, n=6). As shown in panel, B-hCAXII MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-hCAXII MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6), and on 35 days post inoculation, tumor cells were harvested and assessed for human CAXII expression by flow cytometry. As shown, human CAXII was highly expressed on the surface of tumor cells. Therefore, B-hCAXII MC38 cells can be used for in vivo efficacy studies of novel CAXII therapeutics.