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B-hBCMA MC38
Common name
B-hBCMA MC38 Catalog number    310720
Aliases BCM, CD269, TNFRSF13A, TNFRSF17 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description



The human BCMA coding sequence was inserted into the ROSA26 locus in MC38 cells. Human BCMA is highly expressed on the surface of B-hBCMA MC38 cells.



Application


B-hBCMA MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hBCMA MC38 cells. The exogenous promoter and human BCMA coding sequence was inserted into the ROSA26 locus in murine.

Protein expression analysis 


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BCMA expression analysis in B-hBCMA MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hBCMA MC38 cultures were stained with species-specific anti-BCMA antibody. Human BCMA was detected on the surface of B-hBCMA MC38 cells but not wild-type MC38 cells. The 2-B01 clone of B-hBCMA MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hBCMA MC38 cells. B-hBCMA MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Protein expression analysis of tumor cells


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B-hBCMA MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human BCMA expression by flow cytometry. As shown, human BCMA was expressed on the surface of tumor cells. Therefore, B-hBCMA MC38 cells can be used for in vivo efficacy studies of novel BCMA therapeutics.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hBCMA MC38 cells. B-hBCMA MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.