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B-luc A20
Common name

 B-Luc A20

Catalog number    311118
Aliases

Gt(ROSA)26Sor

Disease  Reticulum cell sarcoma
Organism
Mouse
Strain  BALB/cAnN
Tissue types

Lymphoma

Tissue  B lymphocyte

Description 


This B-Luc A20 cell line expresses firefly luciferase as a marker of A20 cells. Fluorescence and luminescence can be observed in B-Luc A20 cells.

Application

B-Luc A20 cell line can be used to evaluate anti-cancer drugs in reticulum cell sarcoma in vivo.


Targeting strategy


Gene targeting strategy for B-Luc A20 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.


In vitro luciferase activity of B-Luc A20 cells


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Luminescence signal intensity of B-Luc A20 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc A20 cells have a strong luminescence signal. Clone No. :2-A10.


Quantitative imaging data & body weight changes


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Tumor growth and in vivo imaging of B-Luc A20 cells. B-Luc A20 cells (5x105) were injected into the tail vein of wild-type BALB/c mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc A20 cells can be used for in vivo efficacy evaluation.


Imaging analysis in vivo


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