| Strain Name |
|
Stock No. | 110148 |
| Common name |
B-CAG-tdTomato cKI mice |
Gene symbol and name | ROSA26、tdTomato |
| Strain of origin |
C57BL/6N |
Chromosome | 6 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
Application
This CAG-tdTomato model is an efficient tool to study the tissue specification of Cre mice, when cross with the Cre mice.
Expression
Expressed gene
tdTomato, Red fluresence protein, report gene
Site of expression
CAG promotor, Extensive expression throughout the body
Gene editing strategy
A CAG-LSL-tdTomato-WPRE-pA
cassette was placed between the exon 1 and exon 2 using C57BL/6 embryonic stem
cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Experimental design : T cells were isolated from peripheral blood in the single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.
Results: tdTomato+ cells were detected in the double-positive mice.
Experimental design : T cells were isolated from spleen in single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.
Results: tdTomato+ cells were detected in the double-positive mice.
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