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B-Pdgfrb-iCre mice
Strain Name

C57BL/6-Pdgfrbtm1(iCre)/Bcgen

Stock No.  110129
Common name

B-Pdgfrb-iCre mice

Gene symbol and name  Pdgfrb(platelet derived growth factor receptor, beta polypeptide), Pdg; CD140b
Strain of origin

C57BL/6

Chromosome  18
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
18596

Application

  • Function research of genes
  • This PdgfrbiCre model is an efficient tool to study the various gene functions when crossed with mice with different loxP site-flanked genes of interest.

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

endothelial cells, central nervous system (CNS) blood vessels


Gene editing strategy


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An F2A-iCre sequence cassette was placed between the coding sequence of exon 23 and 3’UTR of the Pdgfrb gene. This strain was maintained on a C57BL/6 genetic background.


Phenotype Analysis


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(B) Quantification of the number of CD31+ capillaries expressing claudin-5 or ZO-1 and the MFI of claudin-5 or ZO-1 in CD31+ capillaries showed reduction of claudin-5 in capillaries of cortex from Cd146−/− mice compared with those from Cd146+/+ mice at P5 and 4–6 wk. (C) Brain sections from cortex of mice at 4–6 wk were costained for CD31 (green) and PDGFRβ (red) and analyzed by LSFM after being optically cleared by using organic solvents. Representative MIPs of 40 virtual single slices from Cd146+/+ and Cd146−/− mice are shown. (Scale bar: 50 μm.)

(source: Chen J et al.,2017)

reference


1. Olson LE; Soriano P. 2011. PDGFRbeta Signaling Regulates Mural Cell Plasticity and Inhibits Fat Development. Dev Cell 20(6):815-26. [PubMed: 21664579]

2. Chhabra A; Lechner AJ; Ueno M; Acharya A; Van Handel B; Wang Y; Iruela-Arispe ML; Tallquist MD; Mikkola HK. 2012. Trophoblasts regulate the placental hematopoietic niche through PDGF-B signaling. Dev Cell 22(3):651-9. [PubMed: 22387002]

3. Hellstrom M; Kal n M; Lindahl P; Abramsson A; Betsholtz C. 1999. Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development 126(14):3047-55. [PubMed: 10375497]

4. Klinghoffer RA; Mueting-Nelsen PF; Faerman A; Shani M; Soriano P. 2001. The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions. Mol Cell 7(2):343-54. [PubMed: 11239463]

5. Lindahl P; Hellstrom M; Kalen M; Karlsson L; Pekny M; Pekna M; Soriano P; Betsholtz C. 1998. Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli. Development 125(17):3313-22. [PubMed: 9693135]

6. Chen J, Luo Y, Hui H, Cai T, Huang H, Yang F, Feng J, Zhang J, Yan X. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):E7622-E7631. [PubMed: 28827364]