top
Please input keywords
Order
*Country
日本
アメリカ
中国
オーストラリア
シンガポール
イギリス
フランス
ドイツ
スイス
イタリア
カナダ
韓国
オランダ
ベルギー
スウェーデン
その他
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-Htr2c-iCre mice
Strain Name

C57BL/6-Htr2ctm1(iCre)/Bcgen

Stock No.  110139
Common name

B-Htr2c-iCre mice

Gene symbol and name  Htr2c(5-hydroxytryptamine (serotonin) receptor 2C), SR1; Htr1; 5HT1c
Strain of origin

C57BL/6

Chromosome  X
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
15560

Application

  • Function research of genes
  • This Htr2ciCre model is an efficient tool to study the various gene functions when crossed with mice with different loxP site-flanked genes of interest.

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

Biased expression in cerebellum adult, frontal lobe adult.


Gene editing strategy


from clipboard


An P2A-iCre sequence cassette was placed between the coding sequence of exon 6 and 3’UTR of the Htr2c gene. This strain was maintained on a C57BL/6 genetic background.


Phenotype Analysis


from clipboard


Top: a construct was used to generate CaMPARIloxP/loxP mice.

Bottom: low-and high-magnifications images show CaMPARI expression in Htr2c cells after crossing CaMPARIloxP/loxP mice with Htr2c-Cre mice.

(source: Li X et al., 2018)

reference


1. Li X, Chen W, Pan K, Li H, Pang P, Guo Y, Shu S, Cai Y, Pei L, Liu D, Afewerky HK, Tian Q, Zhu LQ, Lu Y. Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nat Neurosci. 2018 Sep;21(9):1239-1250. [PubMed: 30104733]

2. Burke LK, Doslikova B, D'Agostino G, Greenwald-Yarnell M, Georgescu T, Chianese R, Martinez de Morentin PB, Ogunnowo-Bada E, Cansell C, Valencia-Torres L, Garfield AS, Apergis-Schoute J, Lam DD, Speakman JR, Rubinstein M, Low MJ, Rochford JJ, Myers MG, Evans ML, Heisler LK. Sex difference in physical activity, energy expenditure and obesity driven by a subpopulation of hypothalamic POMC neurons. Mol Metab. 2016 Jan 22;5(3):245-252. [PubMed: 26977396]

3. Park S, Williams KW, Liu C, Sohn JW. A neural basis for tonic suppression of sodium appetite. Nat Neurosci. 2020 Mar;23(3):423-432. [PubMed: 31959933]

4. D'Agostino G, Lyons D, Cristiano C, Lettieri M, Olarte-Sanchez C, Burke LK, Greenwald-Yarnell M, Cansell C, Doslikova B, Georgescu T, Martinez de Morentin PB, Myers MG Jr, Rochford JJ, Heisler LK. Nucleus of the Solitary Tract Serotonin 5-HT2C Receptors Modulate Food Intake. Cell Metab. 2018 Oct 2;28(4):619-630.e5. [PubMed: 30146485]