| Strain Name |
|
Stock No. | 110125 |
| Common name |
B-Grpr-iCre mice |
Gene symbol and name | Grpr(gastrin releasing peptide receptor), GR; GRP-R |
| Strain of origin |
C57BL/6 |
Chromosome | X |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
14829 | ||
Application
- Function research of genes
- This GrpriCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of pruritogenic stimuli, long-term potentiation (LTP) and amygdala-dependent memory for fear.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
mouse Grp is highly expressed both in the lateral nucleus of the amygdala, the nucleus where associations for pavlovian learned fear are formed, and in the regions that convey fearful auditory information to the lateral nucleus.
Gene editing strategy
An F2A-iCre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Grpr gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
(I) Schematic depicting virus and retrobeads injection, as well as recording configuration in spinal slices. (J) All recorded cells were filled with biocytin (blue) and were beads-positive (red). GRPR+ fibers were labeled with EYFP (green). Scale bar, 10 mm.
(source: Mu D et al.,2017)
reference
1. Mu D, Deng J, Liu KF, Wu ZY, Shi YF, Guo WM, Mao QQ, Liu XJ, Li H, Sun YG. A central neural circuit for itch sensation. Science. 2017 Aug 18;357(6352):695-699. [PubMed: 28818946]
2. Maslen, G. L., Boyd, Y. 1993. Comparative mapping of the Grpr locus on the X chromosomes of man and mouse. Genomics 17: 106-109. [PubMed: 8406441]
3. Shumyatsky, G. P., Tsvetkov, E., Malleret, G., Vronskaya, S., Hatton, M., Hampton, L., Battey, J. F., Dulac, C., Kandel, E. R., Bolshakov, V. Y. 2002. Identification of a signaling network in lateral nucleus of amygdala important for inhibiting memory specifically related to learned fear. Cell 111: 905-918. [PubMed: 12526815]
4. Sun, Y.G., Zhao, Z.Q., Meng, X.L., Yin, J., Liu, X.Y., Chen, Z.F. Cellular basis of itch sensation. Science 325: 1531-1534, 2009. [PubMed: 19661382]
