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B-Gfap-iCre mice
Strain Name

C57BL/6-Gfaptm1(icre)/Bcgen

Stock No.  110144
Common name

B-Gfap-iCre mice

Gene symbol and name  Gfap (glial fibrillary acidic protein)
Strain of origin

C57BL/6

Chromosome  11
Coat color Black Dietary information Growth and reproduction diet for experimental mice

Description & Application


Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.


Application

This Gfap-iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of astrocytes cells related signaling.


Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

Biased expression in cerebellum adult (RPKM 34.4), frontal lobe adult (RPKM 32.7) and 4 other tissues, astrocyte


Gene editing strategy


from clipboard


A target vector was designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 'UTR downstream of GFAP. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulted chimeric mice. These mice were maintained on a C57BL/6 background.


Phenotype analysis


from clipboard


In situ hybridization in hippocampal CA1 area to confirm µR selective

deletion from µRGFAP-/- mice with probes of µR mRNA (red),

GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).


Reference


1. McCall MA, Gregg RG, Behringer RR, Brenner M, Delaney CL, Galbreath EJ, Zhang CL, Pearce RA, Chiu SY, Messing A. Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6361-6. [PubMed: 8692820]