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B-Drd2-Cre rats
Strain Name

SD-Drd2tm1(Cre)/Bcgen

Stock No.  210488
Common name

B-Drd2-Cre rats

Gene symbol and name  Drd2 (dopamine receptor D2), D2R; Drd-2
Strain of origin

SD

Chromosome  8
Coat color White Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
24318

Application

  • Function research of genes
  • This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction. 

Expression


Expressed gene

Cre, cre recombinase, bacteriophage P1


Site of expression

postsynaptic dopaminergic neurons


Gene editing strategy


from clipboard


A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.


Phenotype Analysis


from clipboard



k Immunofluorescent images of tdTomato and DRD2 in the striatum of Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right, quantifcation results. NS not signifcant, n= 3, t test

(source: Yu Q et al.,2019)

reference


1. Yu Q, Liu YZ, Zhu YB, Wang YY, Li Q, Yin DM. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct. 2019 Apr;224(3):1035-1049. [PubMed: 30604007]

2. Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P., Salon, J., Christie, M., Machida, C. A., Neve, K. A., Civelli, O. Cloning and expression of a rat D2 dopamine receptor cDNA. Nature 336: 783-787, 1988. [PubMed: 2974511]

3. Duan, J., Wainwright, M. S., Comeron, J. M., Saitou, N., Sanders, A. R., Gelernter, J., Gejman, P. V. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Hum. Molec. Genet. 12: 205-216, 2003. [PubMed: 12554675]

4. Basu, S., Nagy, J. A., Pal, S., Vasile, E., Eckelhoefer, I. A., Bliss, V. S., Manseau, E. J., Dasgupta, P. S., Dvorak, H. F., Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nature Med. 7: 569-574, 2001. [PubMed: 11329058]

5. Balk, J.-H., Picetti, R., Salardi, A., Thirlet, G., Dierich, A., Depaulls, A., Le Meur, M., Borrelli, E. Parkinsonian-like locomotor impairment in mice lacking dopamine D2 receptors. Nature 377: 424-428, 1995. [PubMed: 7566118]