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B-Cx3cr1-iCreERT2-EGFP mice
Strain Name

C57BL/6-Cx3cr1tm1(icre/ERT2-EGFP)/Bcgen

Stock No.  110779
Common name

B-Cx3cr1-iCreERT2-EGFP mice

Gene symbol and name  Cx3cr1,chemokine (C-X3-C motif) receptor 1
Strain of origin

C57BL/6

Chromosome  9
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
13051

Application

  • Function research of genes
  • Function research of microglial cells and other Cx3cr1-expressing immune cells

Expression


Expressed gene

iCre/ERT2, improved cre recombinase and estrogen receptor 1 (human) fusion gene

EGFP, enhanced green fluorescent protein


Site of expression

monocytes, macrophage and brain microglia


Gene editing strategy


from clipboard


A targeting vector was designed to replace 390bp of exon 2 of the Cx3cr1 gene with a iCre/ERT2 (improved cre recombinase fulsed to estrogen receptor 1) coding sequence, followed by a porcine teschovirus-1 2A (P2A) and a enhanced green fluorescent protein (EGFP). The targeting vector were injected into C57BL/6N mouse zygotes. After injection, surviving 2-cell-stage zygotes were transplanted to the KM albino pseudopregnant females. The reslulting Cx3cr1-iCreERT2-EGFP founder mice were bred to C57BL/6N mice. These mice were maintained on a C57BL/6N background.


Phenotype Analysis

from clipboard


The expression of EGFP and tdTomato in mouse spleen, bone marrow, and brain mononuclear macrophages was detected by flow cytometry, and Cx3cr1 (Mut /+) was found after induction of Tamoxifen.The majority of macrophages in the brain of tdTomato (Mut/+) mice were double positive cells of EGFP and tdTomato, while the mononuclear macrophages in spleen and bone marrow were single positive cells of EGFP. The results were consistent with the results in the study, indicating that B-Cx3cr1-iCreERT2-EGFP mice were designed and expressed successfully.


Reference


1. GoldmannTobias,WieghoferPeter,Müller Philippe F et al. A new type of microglia gene targeting shows TAK1 to be pivotal in CNS autoimmune inflammation.[J] .Nat. Neurosci., 2013, 16: 1618-26. [PMID: 24077561]

2. YonaSimon,Kim Ki-Wook,Wolf Yochai et al. Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis.[J] .Immunity, 2013, 38: 79-91. [PMID: 23273845]

3. Rogers J T ,Morganti J M , Bachstetter A D , et al. CX3CR1 Deficiency Leads to Impairment of Hippocampal Cognitive Function and Synaptic Plasticity[J]. Journal of Neuroscience, 2011, 31(45):16241-16250. [PMID: 22072675]

4. Ushijima T , Fujimoto N , Matsuyama S , et al. The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice[J]. Journal of Biological Chemistry, 2017:jbc.M117.813931. [PMID: 29158260]

5. Madisen L ,Zwingman T A , Sunkin S M , et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain[J]. NATURE NEUROSCIENCE, 2009, 13(1):133-140. [PMID: 20023653]