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B-hITGA5 mice

Strain Name C57BL/6-Itga5tm1(ITGA5)/Bcgen Common Name  B-hITGA5 mice
Background C57BL/6 Catalog number  111254
Related Genes 
integrin subunit alpha 5, CD49e, FNRA, VLA-5, VLA5A

mRNA expression analysis



Strain specific analysis of ITGA5 gene expression in wild-type mice and B-hITGA5 mice by RT-PCR. 
Mouse Itga5 mRNA was detectable in bladder of wild-type mice (+/+). Human ITGA5 mRNA was detectable only in homozygous B-hITGA5 but not in wild-type mice.


Protein expression analysis 


Strain specific ITGA5 expression analysis in homozygous B-hITGA5 mice by flow cytometry. 
Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGA5 mice (H/H) stimulated with anti-CD3e in vivo (7.5 μg/mice, stimulation for 24 hours, i.p. ), and analyzed by flow cytometry with species-specific anti-ITGA5 antibody. Mouse ITGA5 was exclusively detectable in WT mice. Human ITGA5 were exclusively detectable in homozygous B-hITGA5 but not in wild-type mice.



Strain specific ITGA5 expression analysis in homozygous B-hITGA5 mice by flow cytometry. 
Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGA5 mice (H/H) stimulated with anti-CD3e in vivo (7.5 μg/mice, stimulation for 24 hours, i.p. ), and analyzed by flow cytometry with species-specific anti-ITGA5 antibody. Mouse ITGA5 was exclusively detectable in WT mice. Human ITGA5 were exclusively detectable in homozygous B-hITGA5 but not in wild-type mice.