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B-hIL27/hIL27RA mice
Strain Name C57BL/6-Il27tm1(IL27)Il27ratm1(IL27RA)/Bcgen Common Name  B-hIL27/hIL27RA mice
Background C57BL/6 Catalog number 121566
Related Genes 
IL-27, IL-27AA, IL27p28, IL30, p28, IL27
CRL1, IL-27RA, IL27R, TCCR, WSX1, zcytor1

Protein expression analysis

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Strain specific IL27 expression analysis in homozygous  B-hIL27/hIL27RA mice by ELISA. BMDC culture supernatant was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL27/hIL27RA mice (H/H,H/H) stimulated with LPS(1μg/ml), and analyzed by ELISA with species-specific IL27 ELISA kit. Mouse IL27 was exclusively detectable in wild-type mice. Human IL27 was detectable in homozygous B-hIL27/hIL27RA mice but not in wild-type mice.

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Strain specific IL27RA expression analysis in homozygous B-hIL27/hIL27RA mice by flow cytometry. CD4+ T cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL27/hIL27RA mice (H/H,H/H) stimulated with anti-CD3ε and anti-CD28 in vitro for 48h, and analyzed by flow cytometry with species-specific anti-IL27RA antibody. Mouse IL27RA was exclusively detectable in the wild-type mice. Human IL27RA was detectable in homozygous B-hIL27/hIL27RA mice but not in wild-type mice. 

Intracellular staining of pSTAT1 induced by IL27


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Intracellular pSTAT1 analysis in homozygous B-hIL27/hIL27RA mice by flow cytometry. T cells were isolated from spleen of wild-type mice (+/+) and homozygous B-hIL27/hIL27RA mice (H/H,H/H). CD4+ T cells were purified using magnetic beads then stimulated with mouse or human IL27 in vitro for 15min, and analyzed by flow cytometry with anti-p-STAT1 antibodies. Both mouse and human IL27 can induce STAT1, which means that IL27RA humanized didn’t affect the function of receptor.

Analysis of leukocytes cell subpopulation in spleen

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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, monocytes, dendritic cells, granulocytes and macrophages in homozygous B-hIL27/hIL27RA mice were similar to those in the C57BL/6 mice, demonstrating that IL27/IL27RA humanized does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen

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Analysis of T cell subpopulation in spleen. The lymphocytes were isolated from spleen in C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). The proportion of T cells subpopulation was tested by flow cytometry. There were no differences between C57BL/6 and B-hIL27/hIL27RA mice, demonstrating that humanized of IL27/IL27RA does not change the overall development, differentiation or distribution of these T cell subtypes. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node

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Analysis of lymph node leukocyte subpopulations by FACS. Lymph node were isolated from female C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). Flow cytometry analysis of the lymph node were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hIL27/hIL27RA mice were similar to those in the C57BL/6 mice, demonstrating that IL27/IL27RA humanized does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in lymph node

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Analysis of T cell subpopulation in lymph node. The lymphocytes were isolated from lymph node in C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). The proportion of T cells subpopulation was tested by flow cytometry. There were no differences between C57BL/6 and B-hIL27/hIL27RA mice, demonstrating that humanized of IL27/IL27RA does not change the overall development, differentiation or distribution of these T cell subtypes. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in blood

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Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). Flow cytometry analysis of the blood cells were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, monocytes, dendritic cells, granulocytes and macrophages in homozygous B-hIL27/hIL27RA mice were similar to those in the C57BL/6 mice, demonstrating that IL27/IL27RA humanized does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

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Analysis of T cell subpopulation in blood. The lymphocytes were isolated from blood in C57BL/6 and B-hIL27/hIL27RA mice (n=3, 6-week-old). The proportion of T cells subpopulation was tested by flow cytometry. There were no differences between C57BL/6 and B-hIL27/hIL27RA mice, demonstrating that humanized of IL27/IL27RA does not change the overall development, differentiation or distribution of these T cell subtypes. Values are expressed as mean ± SEM.