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B-hGAS6 mice
Strain Name C57BL/6-Gas6tm1(GAS6)/Bcgen  Common Name  B-hGAS6 mice
Background C57BL/6 Catalog number  110985
Related Genes 
Gas6 (AXSF; AXLLG)

Model description


Gas6 (growth arrest specific 6)  encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. The functions of Gas6 are limited to TAM receptor activation. When activated, the TAM receptors like AXL have effects on primary hemostasis and coagulation and display an anti-inflammatory or a proinflammatory effect, depending on cell type. The Gas6/Axl signaling pathway has been implicated in the promotion of tumor cell proliferation, survival, migration, invasion, angiogenesis, and immune evasion, and are involved in several biological functions and participate in the development and progression of a range of malignancies and autoimmune disorders1. In the tumor microenvironment, cancer-associated fibroblasts (CAFs) and CD45+-expressing tumor-infiltrating leukocytes (TILs) also express Gas6. Among the TILs, macrophages and dendritic cells express high levels of Gas6, Given that Gas6 and Axl are expressed on both neoplastic and host cells, targeting this pathway presents a novel strategy to impair multiple stages of cancer development, progression, and metastasis. A number of Axl inhibitors have been developed and studied in preclinical and clinical settings for various cancer types2.


mRNA expression analysis


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Strain specific analysis of GAS6 gene expression in wild-type C57BL/6 mice and B-hGAS6 mice by RT-PCR. Mouse Gas6 mRNA was detectable only in ovary of wild-type C57BL/6 mice (+/+). Human GAS6 mRNA was detectable only in homozygous B-hGAS6 mice (H/H), but not in wild-type mice. 


Protein expression analysis

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Strain specific GAS6 expression analysis in homozygous B-hGAS6 mice by ELISA. Plasma was collected from wild-type mice (+/+) and homozygous B-hGAS6 mice (H/H), and analyzed by ELISA with species-specific GAS6 ELISA kit. Mouse GAS6 was detectable only in wild-type mice. Human GAS6 was exclusively detectable in homozygous B-hGAS6 mice but not in wild-type mice.


References




[1] Di Stasi R, De Rosa L, D'Andrea LD. Therapeutic aspects of the Axl/Gas6 molecular system. Drug Discov Today. 2020;25(12):2130-2148.
[2] Tanaka M, Siemann DW. Gas6/Axl Signaling Pathway in the Tumor Immune Microenvironment. Cancers (Basel). 2020;12(7):1850. Published 2020 Jul 9. doi:10.3390/cancers12071850 
[3] Shin SA, Moon SY, Park D, Park JB, Lee CS. Apoptotic cell clearance in the tumor microenvironment: a potential cancer therapeutic target. Arch Pharm Res. 2019;42(8):658-671. doi:10.1007/s12272-019-01169-2