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B-hFcRn mice
Strain Name C57BL/6-Fcgrttm1(FCGRT)/Bcgen Common Name  B-hFcRn mice
Background C57BL/6 Catalog number  110001
Related Genes 
FCGRT(Fc fragment of IgG receptor and transporter)
NCBI Gene ID
14132

mRNA expression analysis


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Strain specific analysis of FCGRT gene expression in WT and B-hFcRn mice by RT-PCR.
 Mouse Fcgrt mRNA was detectable in kidney of wild-type (+/+). Human FCGRT mRNA was detectable only in H/H but not in +/+ mice. 


Protein expression analysis


Strain specific analysis of FcRn expression in C57BL/6(+/+) and B-hFcRn mice by western blot.

Kidney was collected from C57BL/6(+/+)  mice  and homozygous B-hFcRn(H/H)  mice and analyzed by western blot with species-specific anti-FcRn antibody. Mouse FcRn was detectable in C57BL/6(+/+) mice. Human FcRn was detectable only in B-hFcRn(H/H)  but not in C57BL/6(+/+) mice.




Serum half-life of Ab1 in B-hFcRn mice



PK profiles in B-hFcRn and C57BL/6 mice IV-dosed at 10 mg/kg with Ab1. Data points from mice with high immune response to the injected antibody, demonstrated by mouse antihuman antibody titers of greater than 1 to 1000 and corresponding to abrupt  PK changes, were excluded in the analyses.




Different type of IgGs pharmacokinetics in B-hFcRn mice



PK profiles in B-hFcRn and C57BL/6 mice IV-dosed at 10 mg/kg with Ab1(IgG1), Ab2(IgG2) and Ab3(IgG3). Data points from mice with high immune response to the injected antibody, demonstrated by mouse antihuman antibody titers of greater than 1 to 1000 and corresponding to abrupt  PK changes, were excluded in the analyses.




The YTE mutation prolongs Abs half-life in B-hFcRn mice