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B-hCTLA4/hGITR mice
Strain Name
C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfrsf18tm1(TNFRSF18)Bcgen/Bcgen
Common Name  B-hCTLA4/hGITR mice
Background C57BL/6 Catalog number  120962
Related Genes 
CTLA4 (cytotoxic T-lymphocyte-associated protein 4)
TNFRSF18
(tumor necrosis factor receptor superfamily, member 18)
NCBI Gene ID
12477,21936


mRNA expression analysis


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Strain specific analysis of CTLA4 and GITR gene expression in WT and B-hCTLA/hGITR mice by RT-PCR. Mouse Ctla4 and Tnfrsf18 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CTLA4 and GITR mRNA was detectable only in H/H, but not in +/+ mice. 


Protein expression analysis


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Strain specific CTLA4 and GITR expression analysis in homozygous B-hCTLA4/hGITR mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCTLA4/hGITR (H/H) mice analyzed by flow cytometry with species-specific anti-CTLA4 antibody or anti-GITR antibody. Mouse CTLA4 and GITR were exclusively detectable in WT mice. Human CTLA4 and GITR  were exclusively detectable in homozygous B-hCTLA4/hGITR  but not WT mice.



Protein expression analysis

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Strain specific CTLA4 and GITR expression analysis in homozygous B-hCTLA4/hGITR mice by flow cytometry. 

Splenocytes were collected from WT and homozygous B-hCTLA4/hGITR (H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice),  and analyzed by flow cytometry with species-specific anti-CTLA4 antibody or anti-GITR antibody. Mouse CTLA4 and GITR were exclusively detectable in WT mice. Human CTLA4 and GITR  were exclusively detectable in homozygous B-hCTLA4/hGITR  but not WT mice.


Combination therapy of anti-human CTLA4 antibody and anti-human GITR antibody


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Antitumor activity of anti-human CTLA4 antibody combined with anti-human GITR antibody in B-hCTLA4/hGITR mice. (A) Anti-human CTLA4 antibody combined with anti-human GITR antibody inhibited MC38-hPD-L1 tumor growth in B-hCTLA4/hGITR mice. Murine colon cancer MC38-hPD-L1 cells were subcutaneously implanted into homozygous B-hCTLA4/hGITR mice (female, 6-7 week-old, n=6). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with BMS986156 (in house) and Yervoy (in house) with doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown in panel A, combination of BMS986156 and Yervoy shows more inhibitory effects than BMS986156 individual group but not obviously in Yervoy individual group, maybe the doses and schedules should be optimization. The B-hCTLA4/hGITR mice provide a preclinical model for in vivo evaluating combination therapy efficacy of hCTLA4 antibodies and hGITR antibodies. Values are expressed as mean ± SEM.