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B-hCD3E/hIL2RA mice
Strain Name C57BL/6-Cd3etm2(CD3E)Il2ratm1(IL2RA) /Bcgen  Common Name  B-hCD3E/hIL2RA mice
Background C57BL/6 Catalog number  121666
Related Genes 
CD3E, IMD18, T3E, TCRE; IL2RA,CD3e molecule; IL2RA, CD25, IDDM10, IL2R, IMD41, TCGFR, p55, interleukin 2 receptor subunit alpha

Gene description:


The CD3E molecule, epsilon encoded by CD3E gene is a polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 complex, a common surface marker on T cells, has important functions not only as an essential component in forming the T cell receptor (TCR)-CD3 complex, but also as an external signal transducer; therefore, the CD3 complex is one of the target molecules to modulate T cell functions. The epsilon polypeptide plays an essential role in T-cell development.

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor. Its affinity for IL2 and cellular function are tightly regulated and vary in different cell types. The high frequency of CD25 on the surface of many different haematological tumour cells is now well established and, apart from its prognostic significance, CD25 may be present on leukaemic stem cells and enable oncogenic signalling pathways in leukaemic cells. Additionally, high CD25 expression in activated circulating immune cells and Tregs is a factor that has already been exploited by IL2 immunotherapies for treatment of tumours and autoimmune disease. The relative clinical safety and efficacy of administering anti-CD25 radioimmunoconjugates and immunotoxins in various haematological tumour indications has been established and clinical trials of a novel CD25-directed antibody drug conjugate are underway.



Protein expression analysis in T cells





Strain specific CD3E expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).



Protein expression analysis in T cells



Strain specific IL2RA expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry. 
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. Mouse IL2RA was detectable in WT mice (+/+). Human IL2RA was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).