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B-hCD3E/h4-1BB mice
Strain Name C57BL/6-Cd3etm2(CD3E)Tnfrsf9tm1(TNFRSF9) /Bcgen Common Name  B-hCD3E/h4-1BB mice
Background C57BL/6 Catalog number  121281
Related Genes 
CD3E,CD3e molecule, IMD18, T3E, TCRE; TNFRSF9, TNF receptor superfamily member 9,4-1BB, CD137, CDw137, ILA

Gene Description


The CD3E molecule, epsilon encoded by CD3E gene is a polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 complex, a common surface marker on T cells, has important functions not only as an essential component in forming the T cell receptor (TCR)-CD3 complex, but also as an external signal transducer; therefore, the CD3 complex is one of the target molecules to modulate T cell functions. The epsilon polypeptide plays an essential role in T-cell development.
TNFRSF9 (Tumor necrosis factor receptor super family, member 9), also called CD137 and 4-1BB, is a co-stimulatory molecule and is mainly expressed on the surface of T, NK and mononuclear cells. CD137 is activated by its ligand CD137L or activating anti-CD137 antibodies, resulting in T cell activation, proliferation and cytokine production. CD137L is expressed on antigen presenting cells such as dendritic cells and macrophages and activated B cells. In vivo studies show that CD137 activation increases anti-tumor immune responses, providing a new target for immunotherapy.


Protein expression analysis


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Strain specific CD3E expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry. 
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .

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Strain specific 4-1BB expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry. 
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H) stimulated with anti-CD3ε antibody in vivo, and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse 4-1BB was detectable in WT mice (+/+). Human 4-1BB was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .