B-hS100A9 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Cytokine Humanized Mice

Text

B-hS100A9 mice

Strain Name	C57BL/6N-S100a9^{tm1(S100A9)Bcgen}/Bcgen	Common Name	B-hS100A9 mice
Background	C57BL/6N	Catalog number	111224
Related Genes	S100A9 (60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100-A9)

mRNA expression analysis

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Strain specific analysis of S100A9 gene expression in wild-type C57BL/6 mice and B-hS100A9 mice by RT-PCR. Mouse S100a9 mRNA was detectable only in bone marrow cells of wild-type C57BL/6 mice (+/+). Human S100A9 mRNA was detectable only in homozygous B-hS100A9 mice (H/H), but not in wild-type mice.

Protein expression analysis

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Strain specific S100A9 expression analysis in homozygous B-hS100A9 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hS100A9 mice (H/H), and analyzed by flow cytometry with species-specific anti-S100A9 antibody. Mouse S100A9 was detectable in wild-type mice. Human S100A9 was exclusively detectable in homozygous B-hS100A9 but not in wild-type mice.