B-hIL2/hIL2RA/hIL2RB mice
| Strain Name |
C57BL/6-Il2tm1(IL2)Bcgen Il2ratm1(IL2RA)Bcgen ll2rbtm2(IL2RB)Bcgen/Bcgen
|
Common Name | B-hIL2/hIL2RA/hIL2RB mice |
| Background | C57BL/6 | Catalog number | 131076 |
|
Related Genes |
IL2 also known as TCGF, lymphokine IL2RA also known as CD25, IDDM10, IL2R, IMD41, TCGFR, p55 IL2RB also known as CD122. |
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|
NCBI Gene ID |
16183,16184,16185 | ||
Protein expression analysis
Strain specific IL2 expression analysis in homozygous B-hIL2/hIL2RA/ hIL2RB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/ hIL2RB mice (H/H) stimulated with anti-mCD3 and anti-mCD28 in vivo, and analyzed by ELISA with species-specific IL2 ELISA kit. Mouse IL2 was detectable in wild-type mice. Human IL2 was exclusively detectable in homozygous B-hIL2/hIL2RA/ hIL2RB mice but not in wild-type mice.(ND: Not detectable)
Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RA antibody. Mouse IL2RA was detectable in T cells of wild-type mice. Human IL2RA was exclusively detectable in T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RA antibody.
Strain specific IL2RB expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RB antibody. Mouse IL2RB was detectable in T cells of wild-type mice. Human IL2RB was exclusively detectable in T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RB antibody.
Analysis of leukocyte subpopulations by FACS. Splenocytes, lymph node and blood were isolated from C57BL/6 and B-hIL2/hIL2RA/hIL2RB mice (female, n=3, 8-week-old). Flow cytometry analysis were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, granulocytes, monocytes in homozygous B-hIL2/hIL2RA/hIL2RB mice were similar to those in the C57BL/6 mice, demonstrating that hIL2/hIL2RA/hIL2RB humanized does not change the overall development, differentiation or distribution of these cell types. Data of lymph node and blood cells were not shown. Values are expressed as mean ± SEM.
Analysis of T cell subpopulations by FACS. Splenocytes, lymph node and blood were isolated from C57BL/6 and B-hIL2/hIL2RA/hIL2RB mice (female, n=3, 8-week-old). Flow cytometry analysis were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells and Tregs in homozygous B-hIL2/hIL2RA/hIL2RB mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL2/hIL2RA/hIL2RB in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes. Data of lymph node and blood cells were not shown. Values are expressed as mean ± SEM.
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