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B-hCD3EDG/hCD20 mice
Strain Name
C57BL/6-Cd3etm2(CD3E)Bcgen Ms4a1tm2(MS4A1)Bcgen/Bcgen  
Common Name  B-hCD3EDG/hCD20 mice
Background C57BL/6 Catalog number 121703
Related Genes 

CD3E, IMD18, T3E, TCRE, CD3e molecule; CD3D, CD3-DELTA, IMD19, T3D, CD3d molecule;  CD3G, CD3-GAMMA, 

IMD17, T3G, CD3g molecule); MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1

mRNA expression analysis

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Strain specific analysis of CD3DG gene expression in wild type (WT) mice and B-hCD3EDG/hCD20 mice by RT-PCR. Mouse Cd3d and Cd3g mRNA were detectable only in thymocytes of WT mice (+/+). Human CD3D and CD3G mRNA were detectable only in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).


Protein expression analysis

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Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hCD20 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hCD20 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).

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Strain specific CD20 expression analysis in homozygous B-hCD3EDG/hCD20 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hCD20 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice (+/+). Human CD20 was exclusively detectable in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).