B-hCCL2 mice
| Strain Name |
C57BL/6N-Ccl2tm1(CCL2)Bcgen/Bcgen |
Common Name | B-hCCL2 mice |
| Background | C57BL/6N | Catalog number | 111102 |
|
Related Genes |
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30 |
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Targeting strategy
Gene targeting strategy for B-hCCL2 mice. The full coding region sequence of mouse Ccl2 was replacement by counterpart human CCL2 sequences in B-hCCL2 mice, so the mouse Ccl2 gene was depleted.
mRNA expression analysis
Strain specific analysis of CCL2 gene expression in wild-type mice and B-hCCL2 mice by RT-PCR. Mouse Ccl2 mRNA was detectable in stomach of wild-type mice (+/+). Human CCL2 mRNA was detectable only in homozygous B-hCCL2 mice (H/H) but not in wild-type C57BL/6 mice (+/+).
Protein expression analysis
Strain specific CCL2 expression analysis in homozygous B-hCCL2 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCCL2 mice (H/H) stimulated with anti-mCD3ε antibody for 4 hours in vivo, and analyzed by ELISA with species-specific CCL2 ELISA kit. Mouse CCL2 were only detectable in wild-type mice. Human CCL2 was exclusively detectable in homozygous B-hCCL2 mice but not in wild-type mice.
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